{"id":1451,"date":"2020-11-12T15:36:57","date_gmt":"2020-11-12T14:36:57","guid":{"rendered":"http:\/\/atlas-parazitov.uvlf.sk\/?p=1451"},"modified":"2020-12-02T20:27:34","modified_gmt":"2020-12-02T19:27:34","slug":"vysetrenie-krvi","status":"publish","type":"post","link":"https:\/\/atlas-parazitov.uvlf.sk\/?p=1451","title":{"rendered":"Vy\u0161etrenie krvi"},"content":{"rendered":"

Nat\u00edvny krvn\u00fd prepar\u00e1t <\/strong>\u2013 pou\u017e\u00edva sa na diagnostiku najm\u00e4 Trypanosoma <\/em>spp. z perif\u00e9rnej krvi<\/p>\n

Tenk\u00fd krvn\u00fd n\u00e1ter<\/strong> \u2013 je vhodn\u00fd na diagnostiku parazitov \u017eij\u00facich v krvi ako napr.: Trypanosoma <\/em>spp., Babesia <\/em>spp., Hepatozoon <\/em>spp., Plasmodium <\/em>spp., Leucocytozoon <\/em>spp. a Haemoproteus <\/em>spp..<\/em> <\/p>\n

Postup: mal\u00e1 kvapka krvi, na odmastenom podlo\u017enom skl\u00ed\u010dku, sa roztrie hranou \u010dist\u00e9ho skl\u00ed\u010dka pod uhlom 45\u00b0 proti smeru n\u00e1teru, nech\u00e1 sa uschn\u00fa\u0165 na vzduchu, fixuje sa metanolom 1 min. a farb\u00ed sa 10% roztokom Giemsom asi 20-30 min. (Krv sa m\u00f4\u017ee odobra\u0165 aj do sk\u00famavky s antikoaluga\u010dn\u00fdm \u010dinidlom EDTA, uchov\u00e1va sa v chlade a do 3 hod\u00edn sa zhotov\u00ed n\u00e1ter.)<\/p>\n

Hrub\u00fd krvn\u00fd n\u00e1ter<\/strong> \u2013 pou\u017e\u00edva sa na diagnostiku najm\u00e4 Trypanosoma <\/em>spp. z perif\u00e9rnej krvi, menej \u010dasto pre Babesia <\/em>spp., Hepatozoon <\/em>spp., Plasmodium <\/em>spp., Leucocytozoon <\/em>spp. a<\/em> Haemoproteus<\/em> spp..<\/p>\n

Postup: mal\u00e1 kvapka krvi sa rozotrie do kruhu o priemere 4 mm a nech\u00e1 sa vysu\u0161i\u0165 na vzduchu a fixuje sa (nie metanolom) pri 80 \u00b0C asi 5 min. a ofarb\u00ed sa 10% roztokom Giemsom asi 15 min.<\/p>\n

Odtla\u010dkov\u00fd prepar\u00e1t<\/strong> \u2013 pou\u017e\u00edva sa na diagnostiku najm\u00e4 Babesia <\/em>spp.<\/p>\n

Postup: prepar\u00e1t sa zhotov\u00ed otla\u010den\u00edm \u010derstvo narezan\u00e9ho org\u00e1nu (mozgov\u00e1 k\u00f4ra, obli\u010dky, pe\u010de\u0148, slezina a kostn\u00e1 dre\u0148) na podlo\u017en\u00e9 skl\u00ed\u010dko alebo roztla\u010den\u00edm malej vzorky tkaniva medzi dvoma podlo\u017en\u00fdmi skl\u00ed\u010dkami, ktor\u00e9 sa rozotrie po d\u013a\u017eke a na oboch podlo\u017en\u00fdch skl\u00ed\u010dkach vznikne jemn\u00fd film, ktor\u00fd sa nech\u00e1 vysu\u0161i\u0165 na vzduchu, fixuje sa absol\u00fatnym metanolom 5 min. a farb\u00ed sa 10% roztokom Giemsom asi 20-30 min.<\/p>\n

Biopsia <\/strong>\u2013 je vhodn\u00e1 na diagnostiku amastigotn\u00fdch \u0161t\u00e1di\u00ed trypanoz\u00f3m, ktor\u00e9 sa vy\u0161etruj\u00fa z bioptick\u00e9ho materi\u00e1lu z prie\u010dne pruhovanej svaloviny alebo myokardu<\/p>\n

S\u00e9rologick\u00e9 met\u00f3dy<\/strong> \u2013 pou\u017e\u00edvaj\u00fa sa na diagnostiku krvn\u00fdch aj tkanivov\u00fdch parazitov (ELISA, KFT, IFAT, IHT, DAT at\u010f.)<\/p>\n","protected":false},"excerpt":{"rendered":"

Nat\u00edvny krvn\u00fd prepar\u00e1t \u2013 pou\u017e\u00edva sa na diagnostiku najm\u00e4 Trypanosoma spp. z perif\u00e9rnej krvi Tenk\u00fd krvn\u00fd n\u00e1ter \u2013 je vhodn\u00fd na diagnostiku parazitov \u017eij\u00facich v krvi ako napr.: Trypanosoma spp., Babesia spp., Hepatozoon spp., Plasmodium spp., Leucocytozoon spp. a Haemoproteus spp..  Postup: mal\u00e1 kvapka krvi, na odmastenom podlo\u017enom skl\u00ed\u010dku, sa roztrie hranou \u010dist\u00e9ho skl\u00ed\u010dka pod uhlom 45\u00b0 proti smeru n\u00e1teru,…<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"ngg_post_thumbnail":0},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/atlas-parazitov.uvlf.sk\/index.php?rest_route=\/wp\/v2\/posts\/1451"}],"collection":[{"href":"https:\/\/atlas-parazitov.uvlf.sk\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/atlas-parazitov.uvlf.sk\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/atlas-parazitov.uvlf.sk\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/atlas-parazitov.uvlf.sk\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1451"}],"version-history":[{"count":3,"href":"https:\/\/atlas-parazitov.uvlf.sk\/index.php?rest_route=\/wp\/v2\/posts\/1451\/revisions"}],"predecessor-version":[{"id":1579,"href":"https:\/\/atlas-parazitov.uvlf.sk\/index.php?rest_route=\/wp\/v2\/posts\/1451\/revisions\/1579"}],"wp:attachment":[{"href":"https:\/\/atlas-parazitov.uvlf.sk\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1451"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/atlas-parazitov.uvlf.sk\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1451"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/atlas-parazitov.uvlf.sk\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1451"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}